BAZ2A Polyclonal antibody

BAZ2A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30984-1-AP

Synonyms

BAZ2A, DKFZp781B109, FLJ13768, FLJ13780, FLJ45876, hWALp3, KIAA0314, TIP5, TTF I interacting protein 5, WALp3

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Validation Data Gallery

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  WB analysis using 30984-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30984-1-AP (BAZ2A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, PC-3 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30984-1-AP targets BAZ2A in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAZ2A fusion protein Ag33963
• Full Name: bromodomain adjacent to zinc finger domain, 2A
• Calculated Molecular Weight: 211 kDa
• Observed Molecular Weight: 250-270 kDa
• GenBank Accession Number: NM_013449.4
• Gene Symbol: BAZ2A
• Gene ID (NCBI): 11176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.