BATF Polyclonal antibody

BATF Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13507-1-AP

Synonyms

SF HT activated gene 2 protein, B-cell-activating transcription factor, BATF1, B-ATF, Basic leucine zipper transcriptional factor ATF-like

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Validation Data Gallery

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  WB analysis of Transfected HEK-293 using 13507-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13507-1-AP (BATF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 13507-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13507-1-AP (BATF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 13507-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BATF antibody (13507-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

13507-1-AP targets BATF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BATF fusion protein Ag4418
• Full Name: basic leucine zipper transcription factor, ATF-like
• Calculated Molecular Weight: 125 aa, 14 kDa
• GenBank Accession Number: BC032294
• Gene Symbol: BATF
• Gene ID (NCBI): 10538
• RRID: AB_10598158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

basic leucine zipper transcription factor (BATF), also named B-cell-activating transcription factor, SF-HT-activated gene 2 protein. AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8(+) dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate to CD8(+) dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8(+) T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs. The molecular mass of BATF is 14kd.

Publications

Species	Application	Title
rat	WB	Life Sci; Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis.; Authors - Tao Yang
mouse	WB	Regul Pept; Atrial natriuretic peptide suppresses Th17 development through regulation of cGMP-dependent protein kinase and PI3K-Akt signaling pathways.; Authors - Ma Libing L