BARD1 Polyclonal antibody

BARD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22964-1-AP

Synonyms

BARD 1, BARD1, BRCA1 associated RING domain 1

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 22964-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22964-1-AP (BARD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 22964-1-AP is BARD1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BARD1 fusion protein Ag18965
• Full Name: BRCA1 associated RING domain 1
• Calculated Molecular Weight: 777 aa, 87 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC126426
• Gene Symbol: BARD1
• Gene ID (NCBI): 580
• RRID: AB_2879190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis; Authors - Yunshang Chen
	WB	Elife; 53BP1 and BRCA1 control pathway choice for stalled replication restart.; Authors - Yixi Xu
human	WB	Molecules; FOXO3a Mediates Homologous Recombination Repair (HRR) via Transcriptional Activation of MRE11, BRCA1, BRIP1, and RAD50; Authors - Gozde Inci