Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 30017-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 30017-1-AP
IP result of anti-BAK (IP:30017-1-AP, 4ug; Detection:30017-1-AP 1:6000) with HEK-293 cells lysate 1640 ug.
IP result of anti-BAK (IP:30017-1-AP, 4ug; Detection:30017-1-AP 1:6000) with HEK-293 cells lysate 1640 ug.
IF Staining of HeLa using 30017-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BAK antibody (30017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BAK antibody (30017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 30017-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP Figures
IP experiment of HEK-293 using 30017-1-AP
IP result of anti-BAK (IP:30017-1-AP, 4ug; Detection:30017-1-AP 1:6000) with HEK-293 cells lysate 1640 ug.
IF/ICC Figures
IF Staining of HeLa using 30017-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BAK antibody (30017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
BAK Polyclonal antibody
Catalog Number
30017-1-AP
Citations
1
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:200-1:800
Applications
WB, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat, duck
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
