BAG5 Polyclonal antibody

• Featured Product
• KD/KO Validated

BAG5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 26628-1-AP

Synonyms

KIAA0873, Bcl-2-associated athanogene 5, BAG-5, BAG 5

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Validation Data Gallery

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  WB analysis of MCF-7 using 26628-1-AP

  WB result of BAG5 antibody (26628-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG5 transfected MCF-7 cells.

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  WB analysis using 26628-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26628-1-AP (BAG5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 26628-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26628-1-AP (BAG5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 26628-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26628-1-AP (BAG5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 26628-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BAG5 antibody (26628-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: MCF-7 cells, SH-SY5Y cells, mouse brain, rat brain
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26628-1-AP targets BAG5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAG5 fusion protein Ag24320
• Full Name: BCL2-associated athanogene 5
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 48-51 kDa
• GenBank Accession Number: BC044216
• Gene Symbol: BAG5
• Gene ID (NCBI): 9529
• RRID: AB_2880578
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Death Discov; Neuromodulation of BAG co-chaperones by HIV-1 viral proteins and H2O2: implications for HIV-associated neurological disorders.; Authors - Michael R Duggan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (03-03-2022)

It have three bands, one band is missing in the KO tissue.

• Applications: Western Blot
• Primary Antibody Dilution: 1: 3000
• Cell Tissue Type: Mouse Heart