BAG3 Recombinant antibody, PBS Only

BAG3 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240860F12

Cat no : 83779-4-PBS

Synonyms

Docking protein CAIR-1, BIS, Bcl-2-binding protein Bis, Bcl-2-associated athanogene 3, BAG-3

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Validation Data Gallery

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  WB analysis using 83779-4-RR (same clone as 83779-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83779-4-RR (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83779-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83779-4-RR (same clone as 83779-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug BAG3 Recombinant antibody (83779-4-RR, Clone:240860F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83779-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83779-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83779-4-RR against Human BAG3 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83779-4-PBS is BAG3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BAG3 fusion protein Ag0956
• Full Name: BCL2-associated athanogene 3
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 74-80 kDa
• GenBank Accession Number: BC006418
• Gene Symbol: BAG3
• Gene ID (NCBI): 9531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.