BAFF Recombinant antibody

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  WB analysis using 84541-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84541-3-RR (BAFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 84541-3-RR

  U-937 cells were subjected to SDS PAGE followed by western blot with 84541-3-RR (BAFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

BAFF Recombinant Antibody for WB, ELISA

Cat No. 84541-3-RR

Clone No.242006E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

242006E4

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Tested Applications

• Positive WB detected in: U-937 cells, Jurkat cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84541-3-RR targets BAFF in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FusionProtein
• Full Name: tumor necrosis factor (ligand) superfamily, member 13b
• Calculated Molecular Weight: 34kd
• Observed Molecular Weight: 32kd, 17kd
• GenBank Accession Number: NM_033622
• Gene Symbol: Tnfsf13b
• Gene ID (NCBI): 24099
• RRID: AB_3672046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9WU72
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAFF (B-cell-activating factor), also called Tnfsf13b (Tumor necrosis factor ligand superfamily member 13B) and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in stimulating B- and T-cell function and the regulation of humoral immunity. A third B-cell-specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response (PMID: 12867412). BAFF cleaved into 2 chains: membrane form is 32-35 kDa; soluble form is 17 kDa.