BACH1 Recombinant antibody

BACH1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4K14

Cat no : 82842-7-RR

Synonyms

BACH-1, BACH 1, 4K14

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Validation Data Gallery

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  WB analysis using 82842-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82842-7-RR (BACH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 82842-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BACH1 antibody (82842-7-RR, Clone: 4K14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, K-562 cells, Raji cells, rat brain tissue, pig brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82842-7-RR targets BACH1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BACH1 fusion protein Ag5128
• Full Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC063307
• Gene Symbol: BACH1
• Gene ID (NCBI): 571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.