BACE2 Polyclonal antibody

BACE2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16321-1-AP

Synonyms

AEPLC, ALP56, Asp 1, ASP1, ASP21, Aspartic like protease 56 kDa, Aspartyl protease 1, BACE2, BAE2, Beta secretase 2, CDA13, CEAP1, Down region aspartic protease, DRAP, Memapsin 1, Theta secretase, UNQ418/PRO852

---

Validation Data Gallery

• 

  WB analysis using 16321-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16321-1-AP (BACE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 16321-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16321-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16321-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16321-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Hela cells, SH-SY5Y cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16321-1-AP targets BACE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BACE2 fusion protein Ag9404
• Full Name: beta-site APP-cleaving enzyme 2
• Calculated Molecular Weight: 518 aa, 56 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC014453
• Gene Symbol: BACE2
• Gene ID (NCBI): 25825
• RRID: AB_2061667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Brain Behav Immun; Elevated β-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer's disease.; Authors - Linbin Dai
mouse	WB	Int J Neurosci; Activating transcription factor 6 reduces Aβ1-42 and restores memory in Alzheimer's disease model mice.; Authors - Yayun Du