BAAT Polyclonal antibody

BAAT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15990-1-AP

Synonyms

BAAT, BACAT, BAT, Glycine N choloyltransferase

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Validation Data Gallery

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  WB analysis of human liver using 15990-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15990-1-AP (BAAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 15990-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15990-1-AP (BAAT Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 15990-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15990-1-AP (BAAT Antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15990-1-AP

  Immunofluorescent analysis of HepG2 cells using 15990-1-AP (BAAT antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human liver tissue
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15990-1-AP targets BAAT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAAT fusion protein Ag8860
• Full Name: bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase)
• Calculated Molecular Weight: 418 aa, 46 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC009567
• Gene Symbol: BAAT
• Gene ID (NCBI): 570
• RRID: AB_2061361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAAT is bile acid-CoA:amino acid N-acyltransferase which involved in in bile acid metabolism. BAAT acts as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids.

Publications

Species	Application	Title
		Toxicol In Vitro; Toxicity and intracellular accumulation of bile acids in sandwich-cultured rat hepatocytes: Role of glycine conjugates.; Authors - Chatterjee Sagnik S