B7-H4 Recombinant antibody, PBS Only

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  WB analysis using 82850-1-RR (same clone as 82850-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82850-1-RR (B7‑H4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82850-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 82850-1-RR (same clone as 82850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82850-1-RR (B7-H4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82850-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 82850-1-RR (same clone as 82850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82850-1-RR (B7-H4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82850-1-PBS in a different storage buffer formulation.

B7-H4 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 82850-1-PBS

Clone No.4C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

VTCN1, B7‑H4, B7H4, B7h.5, B7 homolog 4

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Product Information

82850-1-PBS targets B7-H4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: V-set domain containing T cell activation inhibitor 1
• Calculated Molecular Weight: 282 aa, 31 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: NM_024626
• Gene Symbol: B7-H4
• Gene ID (NCBI): 79679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z7D3
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

B7-H4, also named VTCN1, B7X, or B7S1, is a single-pass type I membrane protein, which contains two immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7-H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7-H4 is over-expressed in many cancers, including breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.