B7‑H4 Polyclonal antibody, PBS Only

B7‑H4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12080-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse liver using 12080-1-AP (same clone as 12080-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 12080-1-AP (same clone as 12080-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 12080-1-AP (same clone as 12080-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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Product Information

12080-1-PBS targets B7‑H4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B7‑H4 fusion protein Ag2712
• Full Name: V-set domain containing T cell activation inhibitor 1
• Calculated Molecular Weight: 282 aa, 31 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC065717
• Gene Symbol: B7-H4
• Gene ID (NCBI): 79679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.