Anti-Mouse B7-H3/CD276 (MJ18), PBS Only

B7-H3/CD276 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

MJ18

Cat no : 65221-1-PBS

Synonyms

Cd276, CD276 antigen, B7RP 2, B7-H3, B7h3

---

Validation Data Gallery

• 

  FC experiment of mouse peritoneal macrophages using 65221-1-Ig (same clone as 65221-1-PBS)

  1X10^6 mouse peritoneal macrophages were surface stained with 1 ug Anti-Mouse CD276 (65221-1-Ig, Clone:MJ18) and FITC anti-rat IgG1 Antibody at dilution 1:100 (red), or unstained (blue). Cells were not fixed. This data was developed using the same antibody clone with 65221-1-PBS in a different storage buffer formulation.

---

Product Information

65221-1-PBS targets B7-H3/CD276 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse IgG2a Fc-CD276 (aa 1-242) fusion protein
• Full Name: CD276 antigen
• GenBank Accession Number: BC019436
• Gene Symbol: Cd276
• Gene ID (NCBI): 102657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD276 (also known as B7-H3) is a type I transmembrane protein expressed on many tissues and cell types. CD276 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, CD276 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).