B7-H3/CD276 Polyclonal antibody

B7-H3/CD276 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 30052-1-AP

Synonyms

CD276, B7-H3, B7H3, B7 homolog 3, B7 H3

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Validation Data Gallery

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  WB analysis using 30052-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HuH-7 using 30052-1-AP

  IP result of anti-B7-H3/CD276 (IP:30052-1-AP, 4ug; Detection:30052-1-AP 1:15000) with HuH-7 cells lysate 840 ug.

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  IHC staining of human lung cancer using 30052-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 30052-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 30052-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 30052-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 30052-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using B7-H3/CD276 antibody (30052-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, LNCaP cells, U2OS cells
• Positive IP detected in: HuH-7 cells
• Positive IHC detected in: human lung cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30052-1-AP targets B7-H3/CD276 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD276 molecule
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: NM_001024736
• Gene Symbol: CD276
• Gene ID (NCBI): 80381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).