CD86 Polyclonal antibody

CD86 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 30691-1-AP

Synonyms

Cd28l2, B7-2, B70, B7.2, B7 2

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Validation Data Gallery

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  WB analysis using 30691-1-AP

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: DC2.4 cells, NR8383 cells, J774A.1 cells, RAW 264.7 cells, mouse spleen tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Product Information

30691-1-AP targets CD86 in WB, IF, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD86 fusion protein Ag33514
• Full Name: CD86 antigen
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_019388
• Gene Symbol: Cd86
• Gene ID (NCBI): 12524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publications

Species	Application	Title
mouse	WB	J Control Release; Reprogramming tumor-associated macrophages by a dually targeted milk exosome system as a potent monotherapy for cancer; Authors - Ying Chen
mouse,rat	WB,IF,FC	Brain Res Bull; Isobavachalcone alleviates ischemic stroke by suppressing HDAC1 expression and improving M2 polarization; Authors - Qiannan Zhang
mouse	WB	Adv Healthc Mater; Legumain-Guided Ferulate-Peptide Self-Assembly Enhances Macrophage-Endotheliocyte Partnership to Promote Therapeutic Angiogenesis After Myocardial Infarction; Authors - Delong Xu