B4GALT6 Polyclonal antibody

• Featured Product
• KD/KO Validated

B4GALT6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20148-1-AP

Synonyms

EC:2.4.1.274, EC:2.4.1.-, Beta4Gal-T6, Beta-1,4-GalTase 6, Beta-1,4-galactosyltransferase 6

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Validation Data Gallery

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  WB analysis of HEK-293 using 20148-1-AP

  WB result of B4GALT6 antibody (20148-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B4GALT6 transfected HEK-293 cells.

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  WB analysis of mouse heart using 20148-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20148-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20148-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20148-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20148-1-AP (B4GALT6 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20148-1-AP (B4GALT6 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 20148-1-AP

  Immunofluorescent analysis of Hela cells, using B4GALT6 antibody 20148-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse kidney tissue, HEK-293 cells, mouse brain tissue
• Positive IHC detected in: human colon tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20148-1-AP targets B4GALT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B4GALT6 fusion protein Ag13686
• Full Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6
• Calculated Molecular Weight: 382 aa, 45 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: BC074884
• Gene Symbol: B4GALT6
• Gene ID (NCBI): 9331
• RRID: AB_10665357
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-1,4-galactosyltransferase 6 (B4GALT6) is a member of the beta 4-galactosyltransferase family that catalyzes the biosynthesis of glycosphingolipids in UDP-dependent manner (PMID: 23744354). B4GALT6 in astrocytes boosts CNS inflammation and promote neurodegeneration during chronic CNS inflammation (PMID: 25216636).

Publications

Species	Application	Title
mouse	IF	Nat Med; Regulation of astrocyte activation by glycolipids drives chronic CNS inflammation.; Authors - Lior Mayo
human	IHC	Biochim Biophys Acta Mol Cell Biol Lipids; Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors.; Authors - Jiřina Procházková