B3GNT5 Polyclonal antibody

B3GNT5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20422-1-AP

Synonyms

Beta3Gn-T5, beta3Gn T5, Beta-1,3-N-acetylglucosaminyltransferase 5, Beta-1,3-Gn-T5, Beta 1,3 Gn T5

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Validation Data Gallery

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  WB analysis of mouse lung using 20422-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20422-1-AP (B3GNT5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20422-1-AP (B3GNT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 20422-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20422-1-AP (B3GNT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 20422-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20422-1-AP (B3GNT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, MCF-7 cells
• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

20422-1-AP targets B3GNT5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B3GNT5 fusion protein Ag14246
• Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
• Calculated Molecular Weight: 378 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC028058
• Gene Symbol: B3GNT5
• Gene ID (NCBI): 84002
• RRID: AB_10694280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B3GNT5 (UDP-GlcNAc:β-Gal β-1,3-N-acetylglucosaminyltransferase 5) is a glycosyltransferase that transfers an N-acetylglucosamine (GlcNAc) from uridine diphosphate (UDP)-GlcNAc to galactose at the nonreducing end of carbohydrate chain. B3GNT5 is required for tumorigenicity of breast cancer and glycosylation of B3GNT5 is critical for its protein stabilization (PMID: 35526049). B3GNT5 was highly expressed in human GBM and glioma cell lines in culture, and was associated with poor patient survival (PMID: 32677340). B3GNT5 was N-glycosylated, and glycosylation of B3GNT5 stabilized its protein for non-glycosylated B3GNT5 protein degraded more rapidly than glycosylated form (PMID: 35526049).

Publications

Species	Application	Title
human	IHC	J Cell Mol Med; MiR-30a-5p inhibits proliferation and metastasis of hydatidiform mole by regulating B3GNT5 through ERK/AKT pathways.; Authors - Zhenzhen Guo
human	WB	Cell Rep; Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins; Authors - Jicheng Yang
human	WB,IHC	iScience; B3GNT5 is a novel marker correlated with malignant phenotype and poor outcome in pancreatic cancer; Authors - Wei Yao ; KD Validated