B3GNT2 Polyclonal antibody

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  WB analysis using 14200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14200-1-AP (B3GNT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

B3GNT2 Polyclonal Antibody for WB, ELISA

Cat No. 14200-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

b3Gal T7, B3GALT7, B3GN T1, B3GN T2, B3GNT, B3GNT 2, B3GNT1, B3GNT2, Beta 1,3 GalTase 7, Beta 1,3 Gn T1, Beta 1,3 Gn T2, Beta 3 Gx T7, Beta3Gal T7, Beta3GalT7, Beta3Gn T1, Beta3Gn T2, BETA3GNT, BGnT 1, BGnT 2

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, SMMC-7721 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14200-1-AP targets B3GNT2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B3GNT2 fusion protein Ag5404
• Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
• Calculated Molecular Weight: 9 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC030579
• Gene Symbol: B3GNT2
• Gene ID (NCBI): 10678
• RRID: AB_3085431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NY97
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-1,3-N-acetylglucosaminyltransferase (B3GNT2) is the most abundant and ubiquitously expressed B3GNT isoform and displays the greatest in vitro activity for N-glycan and O-glycan poly-LacNAc extensionis. B3GNT2 encodes a poly-N-acetyllactosamine synthase that targets >10 ligands and receptors to disrupt interactions between tumor and T cells and reduce T cell activation (PMID: 35338135, 32663509). B3GNT2 also has five predicted N-glycosylation sites at asparagine residues 79, 89, 127, 173, and 219 (PMID: 33229435). Western blots indicated the glycosylated form of B3GNT2 is detected at ~ 67 kDa.