B3GNT2 Polyclonal antibody

B3GNT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14200-1-AP

Synonyms

b3Gal T7, B3GALT7, B3GN T1, B3GN T2, B3GNT, B3GNT 2, B3GNT1, B3GNT2, Beta 1,3 GalTase 7, Beta 1,3 Gn T1, Beta 1,3 Gn T2, Beta 3 Gx T7, Beta3Gal T7, Beta3GalT7, Beta3Gn T1, Beta3Gn T2, BETA3GNT, BGnT 1, BGnT 2

---

Validation Data Gallery

• 

  WB analysis using 14200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14200-1-AP (B3GNT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, SMMC-7721 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14200-1-AP targets B3GNT2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B3GNT2 fusion protein Ag5404
• Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
• Calculated Molecular Weight: 9 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC030579
• Gene Symbol: B3GNT2
• Gene ID (NCBI): 10678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-1,3-N-acetylglucosaminyltransferase (B3GNT2) is the most abundant and ubiquitously expressed B3GNT isoform and displays the greatest in vitro activity for N-glycan and O-glycan poly-LacNAc extensionis. B3GNT2 encodes a poly-N-acetyllactosamine synthase that targets >10 ligands and receptors to disrupt interactions between tumor and T cells and reduce T cell activation (PMID: 35338135, 32663509). B3GNT2 also has five predicted N-glycosylation sites at asparagine residues 79, 89, 127, 173, and 219 (PMID: 33229435). Western blots indicated the glycosylated form of B3GNT2 is detected at ~ 67 kDa.