B3GALT4 Polyclonal antibody

B3GALT4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13490-1-AP

Synonyms

b3Gal T4, B3GALT4, Beta 1,3 GalTase 4, BETA 3 GALT4, beta3Gal T4, beta3GALT4, Gal T2, GalT4

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Validation Data Gallery

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  IHC staining of human pancreas cancer using 13490-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13490-1-AP (B3GALT4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13490-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13490-1-AP (B3GALT4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

13490-1-AP targets B3GALT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B3GALT4 fusion protein Ag4312
• Full Name: UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4
• Calculated Molecular Weight: 378 aa, 42 kDa
• GenBank Accession Number: BC032574
• Gene Symbol: B3GALT4
• Gene ID (NCBI): 8705
• RRID: AB_2063054
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	FASEB J; Functional role of gangliotetraosylceramide in epithelial-to-mesenchymal transition process induced by hypoxia and by TGF-{beta}.; Authors - Guan Feng F
human	WB	Sci Rep; TNFα-signal and cAMP-mediated signals oppositely regulate melanoma- associated ganglioside GD3 synthase gene in human melanocytes.; Authors - Rika Takeuchi