B3GALT2 Polyclonal antibody

B3GALT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14177-1-AP

Synonyms

B3GALT2, Beta 1,3 GalTase 2, beta3Gal T2, BETA3GALT2, GLCT2

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Validation Data Gallery

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  WB analysis of mouse brain using 14177-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14177-1-AP (B3GALT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14177-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14177-1-AP (B3GALT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 14177-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14177-1-AP (B3GALT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14177-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14177-1-AP (B3GALT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14177-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14177-1-AP (B3GALT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14177-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14177-1-AP (B3GALT2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14177-1-AP targets B3GALT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B3GALT2 fusion protein Ag5384
• Full Name: UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2
• Calculated Molecular Weight: 422 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC022507
• Gene Symbol: B3GALT2
• Gene ID (NCBI): 8707
• RRID: AB_11182828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.