AXL Recombinant antibody, PBS Only (Detector)

AXL Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242014C1

Cat no : 84659-3-PBS

Synonyms

UFO, Tyrosine-protein kinase receptor UFO, EC:2.7.10.1, 242014C1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01487-3

  Sandwich ELISA standard curve of MP01487-3, Human Axl Recombinant Matched Antibody Pair - PBS only. 84659-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1618. 84659-3-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

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  Sample test of MP01487-3

  Serum of six individual healthy human donors was measured. The human Axl concentration of detected samples was determined to be 38,035.1 pg/mL with a range of 26,053.7 - 60,164.0 pg/mL.

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  Sample test of MP01487-3

  Saliva of six individual healthy human donors was measured. The human Axl concentration of detected samples was determined to be 139.3 pg/mL with a range of 70.3 - 202.4 pg/mL.

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  Sample test of MP01487-3

  Urine of six individual healthy human donors was measured. The human Axl concentration of detected samples was determined to be 80,064.8 pg/mL with a range of 37,803.8 - 140,502.0 pg/mL.

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  Sample test of MP01487-3

  U-87 MG human glioblastoma/astrocytoma cells were cultured in MEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and 1 mM sodium pyruvate, until confluent. An aliquot of the cell culture supernate was removed, assayed for human Axl, and measured 1,712.6 pg/mL.

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  Sample test of MP01487-3

  Cerebrospinal fluid of one human donor was measured. The Axl concentration of detected samples was determined to be 2,138.1 pg/mL.

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  Cytometric bead array standard curve of MP01487-1

  Cytometric bead array standard curve of MP01487-1, AXL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84659-2-PBS. Detection antibody: 84659-3-PBS. Standard: Eg1618. Range: 0.313-40 ng/mL.

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Product Information

84659-3-PBS targets AXL as part of a matched antibody pair:

MP01487-1: 84659-2-PBS capture and 84659-3-PBS detection (validated in Cytometric bead array)

MP01487-3: 84659-5-PBS capture and 84659-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: AXL receptor tyrosine kinase
• Calculated Molecular Weight: 98kDa
• GenBank Accession Number: NM_021913.4
• Gene Symbol: AXL
• Gene ID (NCBI): 558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.