Arp5/ACTR5 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Arp5/ACTR5 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11C1

Cat no : 67326-1-PBS

Synonyms

Actin related protein 5, ACTR5, Arp5, Arp5/ACTR5, FLJ12785, hARP5, INO80M, Sarcoma antigen NY SAR 16

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Validation Data Gallery

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  WB analysis using 67326-1-Ig (same clone as 67326-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67326-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67326-1-Ig (same clone as 67326-1-PBS)

  WB result of Arp5/ACTR5 antibody (67326-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells. This data was developed using the same antibody clone with 67326-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C2C12 using 67326-1-Ig (same clone as 67326-1-PBS)

  C2C12 cells were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67326-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig skeletal muscle using 67326-1-Ig (same clone as 67326-1-PBS)

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67326-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 67326-1-Ig (same clone as 67326-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67326-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67326-1-PBS targets Arp5/ACTR5 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Arp5/ACTR5 fusion protein Ag15976
• Full Name: ARP5 actin-related protein 5 homolog (yeast)
• Calculated Molecular Weight: 607 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC038402
• Gene Symbol: ACTR5
• Gene ID (NCBI): 79913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)