Arfaptin-1 Monoclonal antibody, PBS Only

Arfaptin-1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A10B3

Cat no : 67461-1-PBS

Synonyms

Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521

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Validation Data Gallery

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  WB analysis using 67461-1-Ig (same clone as 67461-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67461-1-Ig (same clone as 67461-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67461-1-Ig (same clone as 67461-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67461-1-Ig (same clone as 67461-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67461-1-PBS targets Arfaptin-1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Arfaptin-1 fusion protein Ag12097
• Full Name: ADP-ribosylation factor interacting protein 1
• Calculated Molecular Weight: 373 aa, 42 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC103759
• Gene Symbol: Arfaptin-1
• Gene ID (NCBI): 27236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.