Mouse Apoe Recombinant antibody, PBS Only

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  WB analysis using 83728-4-RR (same clone as 83728-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83728-4-RR (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83728-4-RR (same clone as 83728-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apoe antibody (83728-4-RR, Clone: 240786C3 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83728-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83728-4-RR against Mouse Apolipoprotein E were performed. The affinity constant is 0.793 nM.

Apoe {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83728-4-PBS

Clone No.240786C3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

apolipoprotein E, AI255918, 240786C3

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Product Information

83728-4-PBS targets Apoe in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: Apolipoprotein E
• Calculated Molecular Weight: 36kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: NM_001305819.1
• Gene Symbol: Apoe
• Gene ID (NCBI): 11816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P08226
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Apolipoprotein E (Apoe) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).