Annexin A4 Monoclonal antibody

Annexin A4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10B5

Cat no : 60288-2-Ig

Synonyms

35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV

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Validation Data Gallery

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  WB analysis of COLO 320 using 60288-2-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 60288-2-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 60288-2-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 60288-2-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 60288-2-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60288-2-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60288-2-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60288-2-Ig(Annexin IV antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: COLO 320 cells, human placenta tissue, A549 cells, human heart tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60288-2-Ig targets Annexin A4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin A4 fusion protein Ag0130
• Full Name: annexin A4
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000182
• Gene Symbol: Annexin A4
• Gene ID (NCBI): 307
• RRID: AB_2881405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.