Annexin A3 Monoclonal antibody

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  WB analysis using 66405-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 66405-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66405-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66405-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66405-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 66405-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 66405-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 40x lens).

Annexin A3 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66405-1-Ig

Clone No.1A11E4

Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

ANXA3, 1A11E4, 35 alpha calcimedin, 35-alpha calcimedin, Annexin 3

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, HepG2 cells, L02 cells, Caco-2 cells, human heart tissue, human spleen tissue, SW 1990 cells, DU 145 cells, SCaBER cells, HeLa cells, MCF-10A cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

66405-1-Ig targets Annexin A3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin A3 fusion protein Ag2381
• Full Name: annexin A3
• Calculated Molecular Weight: 322 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000871
• Gene Symbol: Annexin A3
• Gene ID (NCBI): 306
• RRID: AB_2881779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12429
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.

Publications

Species	Application	Title
mouse	WB,IHC,IF	Oncogene; Epstein-Barr virus causes vascular abnormalities in epithelial malignancies through upregulating ANXA3-HIF-1α-VEGF pathway; Authors - Yuanyuan Chen ; KO Validated