Annexin A3 Monoclonal antibody
Annexin A3 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human and More (1)
Applications
WB, IHC, ELISA, IF
Conjugate
Unconjugated
CloneNo.
1A11E4
Cat no : 66405-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HEK-293 cells, L02 cells, human heart tissue, human spleen tissue |
Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
66405-1-Ig targets Annexin A3 in WB, IHC, ELISA, IF applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Annexin A3 fusion protein Ag2381 |
Full Name | annexin A3 |
Calculated Molecular Weight | 322 aa, 36 kDa |
Observed Molecular Weight | 36 kDa |
GenBank Accession Number | BC000871 |
Gene Symbol | Annexin A3 |
Gene ID (NCBI) | 306 |
RRID | AB_2881779 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Annexin A3 antibody 66405-1-Ig | Download protocol |
IHC protocol for Annexin A3 antibody 66405-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |