CoraLite® Plus 488-conjugated Annexin A2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Annexin A2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

3H1B11

Cat no : CL488-60051

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II

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Validation Data Gallery

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  IF Staining of MCF-7 using CL488-60051

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 Annexin A2 antibody (CL488-60051, Clone: 3H1B11 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-60051 targets Annexin A2 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin A2 fusion protein Ag1777
• Full Name: annexin A2
• Calculated Molecular Weight: 339 aa, 39 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC016774
• Gene Symbol: Annexin A2
• Gene ID (NCBI): 302
• RRID: AB_2883091
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Publications

Species	Application	Title
human	IF	Cancer Sci; GNAQ T96S mutation abrogates the ability of wild-type GNAQ to induce apoptosis by phosphorylating annexin A2 in natural killer/T cell lymphoma.; Authors - Wugan Zhao