Annexin A2 Monoclonal antibody, PBS Only

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  WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66035-1-Ig (same clone as 66035-1-PBS)

  WB result of Annexin A2 antibody (66035-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66035-1-Ig (same clone as 66035-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66035-1-Ig (ANXA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66035-1-Ig (same clone as 66035-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66035-1-Ig (ANXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66035-1-Ig (same clone as 66035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66035-1-Ig (same clone as 66035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

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  IF Staining of T-47D using 66035-1-Ig (same clone as 66035-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66035-1-Ig (same clone as 66035-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66035-1-Ig (same clone as 66035-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Annexin A2 Monoclonal antibody (66035-1-Ig, Clone:1C1E12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 66035-1-PBS

Clone No.1C1E12

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II

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Product Information

66035-1-PBS targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin A2 fusion protein Ag16654
• Full Name: annexin A2
• Calculated Molecular Weight: 339 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC016774
• Gene Symbol: Annexin A2
• Gene ID (NCBI): 302
• ENSEMBL Gene ID: ENSG00000182718
• RRID: AB_11045659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07355
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.