Annexin A13 Polyclonal antibody

Annexin A13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25153-1-AP

Synonyms

Annexin 13, annexin A13, Annexin XIII, ANX13, ANXA13, Intestine specific annexin, ISA

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Validation Data Gallery

• 

  WB analysis of mouse small intestine using 25153-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 25153-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 25153-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 25153-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 25153-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse small intestine tissue
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25153-1-AP is Annexin A13 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Annexin A13 fusion protein Ag18581
• Full Name: annexin A13
• Calculated Molecular Weight: 316 aa, 35 kDa
• Observed Molecular Weight: 35 kDa / 40 kDa
• GenBank Accession Number: BC125158
• Gene Symbol: Annexin A13
• Gene ID (NCBI): 312
• RRID: AB_2879928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.