Amylin Polyclonal antibody

Amylin Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 22305-1-AP

Synonyms

Amylin, DAP, Diabetes associated peptide, IAP, IAPP, islet amyloid polypeptide

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Validation Data Gallery

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  IHC staining of human pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22305-1-AP (Amylin Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22305-1-AP (Amylin Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human pancreas tissue, mouse pancreas tissue, rat pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22305-1-AP targets Amylin in IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: islet amyloid polypeptide
• Calculated Molecular Weight: 10 kDa
• GenBank Accession Number: NM_000415
• Gene Symbol: Amylin
• Gene ID (NCBI): 3375
• RRID: AB_2879067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IAPP, also known as Amylin, is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring a tumor of the pancreas that is derived from beta cells and secretes INS. IAPP is co-secreted with INS by pancreatic β-cells and largely contributes to glycemic control. Studies suggest that this protein, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes.