Amylase Alpha Monoclonal antibody, PBS Only

Amylase Alpha Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F1G8

Cat no : 66133-1-PBS

Synonyms

AMY2A, Amylase, alpha, AMY2B, AMY2, 2F1G8

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Validation Data Gallery

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  WB analysis of human saliva using 66133-1-Ig (same clone as 66133-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human saliva using 66133-1-Ig (same clone as 66133-1-PBS)

  0.1 μL human saliva lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig pancreas using 66133-1-Ig (same clone as 66133-1-PBS)

  0.6 μg pig pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat pancreas using 66133-1-Ig (same clone as 66133-1-PBS)

  0.6 μg rat pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66133-1-Ig (same clone as 66133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66133-1-Ig (same clone as 66133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

• 

  IF Staining of BxPC-3 using 66133-1-Ig (same clone as 66133-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Amylase Alpha antibody (66133-1-Ig, Clone: 2F1G8 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

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Product Information

66133-1-PBS targets Amylase Alpha in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Amylase Alpha fusion protein Ag8819
• Full Name: amylase, alpha 2A (pancreatic)
• Calculated Molecular Weight: 511 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC007060
• Gene Symbol: Amylase Alpha
• Gene ID (NCBI): 279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.