Alpha Actinin Recombinant antibody

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  WB analysis using 86340-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86340-2-RR (Alpha Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Alpha Actinin Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86340-2-RR

Clone No.250833F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1

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Tested Applications

• Positive WB detected in: HeLa cells, PANC-1 cells, NIH/3T3 cells, C6 cells, mouse spleen tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86340-2-RR targets Alpha Actinin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Alpha Actinin fusion protein Ag1859
• Full Name: actinin, alpha 1
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC015766
• Gene Symbol: Alpha Actinin
• Gene ID (NCBI): 87
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12814
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.