Alpha 2-Antiplasmin Polyclonal antibody

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  WB analysis using 28549-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 28549-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 28549-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 28549-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28549-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-antiplasmin antibody (28549-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Alpha 2-Antiplasmin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 28549-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Alpha-2-antiplasmin, Serpin F2, ALPHA 2 PI, Alpha 2 AP, Alpha 2 antiplasmin

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Tested Applications

• Positive WB detected in: HepG2 cells, THP-1 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28549-1-AP targets Alpha 2-Antiplasmin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha 2-Antiplasmin fusion protein Ag29670
• Full Name: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
• Calculated Molecular Weight: 491 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC031592
• Gene Symbol: Alpha-2-antiplasmin
• Gene ID (NCBI): 5345
• RRID: AB_2918174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08697
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINF2, also termed Alpha-2-antiplasmin(a2-AP), a member of the serpin family of serine protease inhibitors. SERPINF2 is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in SERPINF2 result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis.