Alpha 1B-Glycoprotein Monoclonal antibody, PBS Only

Alpha 1B-Glycoprotein Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D4E5

Cat no : 66260-1-PBS

Synonyms

A1B, A1BG, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein, Alpha 1B-Glycoprotein, GAB, HYST2477

---

Validation Data Gallery

• 

  WB analysis of human plasma using 66260-1-Ig (same clone as 66260-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66260-1-Ig (Alpha 1B-Glycoprotein antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66260-1-Ig (same clone as 66260-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66260-1-Ig (Alpha 1B-Glycoprotein antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66260-1-Ig (same clone as 66260-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66260-1-Ig (same clone as 66260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66260-1-Ig (same clone as 66260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66260-1-Ig (same clone as 66260-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66260-1-PBS targets Alpha 1B-Glycoprotein in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha 1B-Glycoprotein fusion protein Ag5414
• Full Name: alpha-1-B glycoprotein
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC035719
• Gene Symbol: A1BG
• Gene ID (NCBI): 1
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).