Alpha 1B-Glycoprotein Polyclonal antibody, PBS Only

Alpha 1B-Glycoprotein Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14181-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human plasma using 14181-1-AP (same clone as 14181-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 14181-1-AP (same clone as 14181-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 14181-1-AP (same clone as 14181-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14181-1-AP (same clone as 14181-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hepatocirrhosis using 14181-1-AP (same clone as 14181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hepatocirrhosis using 14181-1-AP (same clone as 14181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14181-1-AP (same clone as 14181-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:25 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

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Product Information

14181-1-PBS targets Alpha 1B-Glycoprotein in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha 1B-Glycoprotein fusion protein Ag5414
• Full Name: alpha-1-B glycoprotein
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC035719
• Gene Symbol: A1BG
• Gene ID (NCBI): 1
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).