Alpha 1 Antitrypsin Monoclonal antibody, PBS Only

Alpha 1 Antitrypsin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

1A9G6

Cat no : 66135-1-PBS

Synonyms

a1-antitrypsin, Alpha-1-antitrypsin, Serpin A1, SERPINA1, AAT

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Validation Data Gallery

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  WB analysis using 66135-1-Ig (same clone as 66135-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66135-1-Ig (same clone as 66135-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66135-1-Ig (same clone as 66135-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66135-1-Ig (same clone as 66135-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66135-1-Ig (same clone as 66135-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

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  WB analysis of human saliva using 66135-1-Ig (same clone as 66135-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 66135-1-Ig (same clone as 66135-1-PBS)

  IP result of anti-Alpha 1 Antitrypsin (IP:66135-1-Ig, 5ug; Detection:66135-1-Ig 1:1000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66135-1-Ig(Alpha-1-Antitrypsin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66135-1-Ig(Alpha-1-Antitrypsin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha 1 Antitrypsin antibody (66135-1-Ig, Clone: 1A9G6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Alpha 1 Antitrypsin (66135-1-Ig, Clone:1A9G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

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Product Information

66135-1-PBS targets Alpha 1 Antitrypsin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha 1 Antitrypsin fusion protein Ag9516
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
• Calculated Molecular Weight: 418 aa, 47 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC015642
• Gene Symbol: Alpha 1-Antitrypsin
• Gene ID (NCBI): 5265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.