Ajuba Polyclonal antibody

Ajuba Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28686-1-AP

Synonyms

Ajuba, JUB, Protein ajuba

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Validation Data Gallery

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  WB analysis using 28686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28686-1-AP (Ajuba antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: mouse skin tissue, human thyroid cancer tissue, mouse small intestine tissue, mouse testis tissue, rat skin tissue, rat small intestine tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28686-1-AP targets Ajuba in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ajuba fusion protein Ag29725
• Full Name: jub, ajuba homolog (Xenopus laevis)
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC007580
• Gene Symbol: Ajuba
• Gene ID (NCBI): 84962
• RRID: AB_2881194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ajuba belongs to the LIM domain proteins, which are involved in the assembly of the extracellular matrix and, along with associated proteins, regulate target genes that connect the extracellular matrix and the cytoskeleton. Ajuba consists of three LIM domains at the C-terminal and is located in the cytoplasm where it interacts with a variety of other proteins. Ajuba contributes to the formation or strengthening of cell-cell adhesion and regulates cell growth and differentiation decisions. Ajuba participates in the assembly of the extracellular matrix. Ajuba also plays an important role in some diseases.

Publications

Species	Application	Title
human	WB	Cell Rep; ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression; Authors - Yu Zhang