Adiponectin Recombinant antibody

Adiponectin Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240392D6

Cat no : 83525-2-RR

Synonyms

Adipoq, Acrp30, Acdc, 240392D6

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Validation Data Gallery

• 

  WB analysis of mouse adipose using 83525-2-RR

  mouse adipose tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adipoq antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat adipose using 83525-2-RR

  rat adipose tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adiponectin antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 83525-2-RR

  rat heart tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adipoq antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83525-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83525-2-RR against Rat Adiponectin were performed. The affinity constant is 0.149 nM.

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Tested Applications

• Positive WB detected in: rat adipose tissue, rat heart tissue, mouse adipose tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:15000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83525-2-RR is Adiponectin Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: adiponectin, C1Q and collagen domain containing
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: NM_144744.3
• Gene Symbol: Adiponectin
• Gene ID (NCBI): 246253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.