Adiponectin receptor 1 Polyclonal antibody

Adiponectin receptor 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22425-1-AP

Synonyms

ADIPOR1, Adiponectin receptor protein 1, PAQR1, CGI-45, CGI45

---

Validation Data Gallery

• 

  WB analysis using 22425-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22425-1-AP (Adiponectin receptor 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 22425-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 22425-1-AP (Adiponectin receptor 1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 22425-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 22425-1-AP (Adiponectin receptor 1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22425-1-AP targets Adiponectin receptor 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Adiponectin receptor 1 fusion protein Ag18187
• Full Name: adiponectin receptor 1
• Calculated Molecular Weight: 375 aa, 43 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC010743
• Gene Symbol: Adiponectin receptor 1
• Gene ID (NCBI): 51094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin have been identified: adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2). They are seven-transmembrane domain receptors and mediate increased AMPK, MAPK and PPARA ligand activity in response to adiponectin. AdipoR1 is found in many tissues, being particularly abundant in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver (PMID: 24417942). AdipoR1 has a high affinity for globular adiponectin and a very low affinity for full-length adiponectin. AdipoR2 has an intermediate affinity for both forms.