Acetyl-Histone H3 (Lys18) Recombinant antibody

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  WB analysis of HSC-T6 using 82832-1-RR

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 82832-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 82832-1-RR

  Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Dot Blot experiment of peptide using 82832-1-RR

  Dot blot analysis was used to confirm the specificity of 82832-1-RR Acetyl-Histone H3 (Lys18) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H3AK18Ac. Column 2: Unmodified H3AK18. Column 3: H3AK36Ac. Column 4: Unmodified H3AK36. Column 5: H3AK23Ac. Column 6: Unmodified H3AK23. Column 7: H3AK122Ac. Column 8:Unmodified H3AK122. Column 9: H3AK27Ac. Column 10: Unmodified H3AK27. Column 11: H3AK14Ac. Column 12: Unmodified H3AK14. Column 13: H3AK56Ac. Column 14:Unmodified H3AK56. Column 15:H3AK9Ac. Column 16:Unmodified H3AK9. Column 17:H3AK4Ac. Column 18:Unmodified H3AK4. Column 19:Blank(PBS).

Acetyl-Histone H3 (Lys18) Uni-rAb^TM Recombinant Antibody for WB, Dot Blot, ELISA

Cat No. 82832-1-RR

Clone No.2E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Dot Blot, ELISA

Synonyms

Acetyl Histone H3, Ac Histone H3-Lys18, Ac Histone H3 Lys18, Ac Histone H3 (Lys18), Ac Histone H3

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Tested Applications

• Positive WB detected in: Trichostatin A treated NIH/3T3 cells, HSC-T6 cells, NIH/3T3 cells
• Positive Dot Blot detected in: peptide

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82832-1-RR targets Acetyl-Histone H3 (Lys18) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• RRID: AB_3670568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.