Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84549-1-RR (Acetyl-Histone H2B (Lys20) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84549-1-RR (Acetyl-Histone H2B (Lys20) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
IF Staining of HeLa using 84549-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Dot Blot experiment of peptide using 84549-1-RR
Dot blot analysis was used to confirm the specificity of 84549-1-RR Acetyl-Histone H2B (Lys20) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.
Dot blot analysis was used to confirm the specificity of 84549-1-RR Acetyl-Histone H2B (Lys20) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.
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Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84549-1-RR (Acetyl-Histone H2B (Lys20) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
IF/ICC Figures
IF Staining of HeLa using 84549-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
DOT BLOT Figures
Dot Blot experiment of peptide using 84549-1-RR
Dot blot analysis was used to confirm the specificity of 84549-1-RR Acetyl-Histone H2B (Lys20) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
