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AbFlex Histone H3K27me3 antibody (rAb)

Host / Isotype

/ IgG2a

Reactivity

Human, Wide Range Predicted

Applications

ChIP, DB, WB

Activ Motif logo

Cat No : 91403,91404 91403

Synonyms

AbFlex, histone H3, histone, H3, histone-H3, histoneH3, pAb, polyclonal, H3K27me3, Histone H3 trimethyl Lys27, Histone H3 trimethyl Lysine 27, Histone H3 trimethyl Lys 27, Histone H3 trimethyl K27, Histone H3 trimethyl K 27, Histone H3 tri-methyl Lys27, Histone H3 tri-methyl Lysine 27, Histone H3 tri-methyl Lys 27, Histone H3 tri-methyl K27, Histone H3 tri-methyl K 27, Histone H3 3Me Lys27, Histone H3 3Me Lysine 27, Histone H3 3Me Lys 27, Histone H3 3Me K27, Histone H3 3Me K 27, Histone H3 Me3 Lys27, Histone H3 Me3 Lysine 27, Histone H3 Me3 Lys 27, Histone H3 Me3 K27, Histone H3 Me3 K 27

Validation Data Gallery

  • AbFlex Histone H3K27me3 antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 17 ug of B2B cell chromatin and 5 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 14 million sequence tags were mapped to identify Histone H3K27me3 binding sites on chromosome 17.

    AbFlex Histone H3K27me3 antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 17 ug of B2B cell chromatin and 5 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 14 million sequence tags were mapped to identify Histone H3K27me3 binding sites on chromosome 17.

  • AbFlex Histone H3K27me3 antibody specificity is shown by Western Blot. 20 ug of HeLa cell nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H3K27me3 antibody at 0.5 ug/ml.

    AbFlex Histone H3K27me3 antibody specificity is shown by Western Blot. 20 ug of HeLa cell nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H3K27me3 antibody at 0.5 ug/ml.

  • AbFlex Histone H3K27me3 antibody specificity is shown by Dot Blot. Recombinant proteins were spotted onto Nitrocellulose as follows: Lane 1 - Histone H3K27me3 (MLA), Cat. No. 31216; Lane 2 - Histone H3K27me2 (MLA), Cat. No. 31215; Lane 3 - Histone H3K27me1 (MLA)Cat. No. 31214; Lane 4 - Histone H3 (C110A), Cat. No. 31207; Probed with AbFlex Histone H3K27me3 antibody at 1 ug/ml.

    AbFlex Histone H3K27me3 antibody specificity is shown by Dot Blot. Recombinant proteins were spotted onto Nitrocellulose as follows: Lane 1 - Histone H3K27me3 (MLA), Cat. No. 31216; Lane 2 - Histone H3K27me2 (MLA), Cat. No. 31215; Lane 3 - Histone H3K27me1 (MLA)Cat. No. 31214; Lane 4 - Histone H3 (C110A), Cat. No. 31207; Probed with AbFlex Histone H3K27me3 antibody at 1 ug/ml.

Product Information

Tested Applications ChIP, DB, WB

Applications Validated by Active Motif: ChIP-Seq: 5 - 10 ug each WB: 0.5 - 2 ug/ml DB: 1 ug/ml
Tested Reactivity Human, Wide Range Predicted
Host / Isotype / IgG2a
Class Recombinant
Type Antibody
ModificationMethylated
Immunogen This Histone H3 trimethyl Lys27 antibody was raised against a peptide containing trimethyl Lys27 of human Histone H3.
Full Name AbFlex Histone H3K27me3 antibody (rAb)
Synonyms AbFlex, histone H3, histone, H3, histone-H3, histoneH3, pAb, polyclonal, H3K27me3, Histone H3 trimethyl Lys27, Histone H3 trimethyl Lysine 27, Histone H3 trimethyl Lys 27, Histone H3 trimethyl K27, Histone H3 trimethyl K 27, Histone H3 tri-methyl Lys27, Histone H3 tri-methyl Lysine 27, Histone H3 tri-methyl Lys 27, Histone H3 tri-methyl K27, Histone H3 tri-methyl K 27, Histone H3 3Me Lys27, Histone H3 3Me Lysine 27, Histone H3 3Me Lys 27, Histone H3 3Me K27, Histone H3 3Me K 27, Histone H3 Me3 Lys27, Histone H3 Me3 Lysine 27, Histone H3 Me3 Lys 27, Histone H3 Me3 K27, Histone H3 Me3 K 27
Molecular weight 17 kDa
Purification Method Protein A Chromatography
Buffer 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.AbFlex Histone H3K27me3 antibody (rAb) was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.