AZI1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AZI1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 25735-1-AP

Synonyms

5 azacytidine induced 1, AZ1, AZI1, Centrosomal protein of 131 kDa, Cep131, KIAA1118

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Validation Data Gallery

• 

  WB analysis of mouse testis using 25735-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25735-1-AP (AZI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 25735-1-AP

  Immunofluorescent analysis of HeLa cells using 25735-1-AP (AZI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

The immunogen of 25735-1-AP is AZI1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AZI1 fusion protein Ag22699
• Full Name: 5-azacytidine induced 1
• Calculated Molecular Weight: 1083 aa, 122 kDa
• Observed Molecular Weight: 131 kDa
• GenBank Accession Number: BC136578
• Gene Symbol: AZI1
• Gene ID (NCBI): 22994
• RRID: AB_2880216
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.

Publications

Species	Application	Title
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human	WB,IF,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li ; KD Validated
human,mouse	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall
human	IF	PLoS Biol; Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium.; Authors - Özge Z Aydin
human	WB	Front Oncol; USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.; Authors - Catherine M Nickson ; KD Validated
human	IF	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jay (Verified Customer) (10-15-2019)

Methanol fixation,Immunostaining with Azi1(red), centrosome marker gamma-tubulin(green) and DAPI(blue) for nucleus.First time to show IF result using mouse NIH3T3 cell line.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse NIH3T3

AZI1 Antibody Immunofluorescence, validation (1:100 dilution) in Mouse NIH3T3 (Cat no:25735-1-AP)