Zinc Alpha 2 Glycoprotein Polyclonal antibody

Zinc Alpha 2 Glycoprotein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13399-1-AP

Synonyms

AZGP1, Zinc-alpha-2-glycoprotein, Zn alpha 2 GP, Zn alpha 2 glycoprotein, ZAG

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Validation Data Gallery

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  WB analysis of human plasma using 13399-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 13399-1-AP (Zinc-alpha-2-glycoprotein antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13399-1-AP

  human plasma was subjected to SDS PAGE followed by western blot with 13399-1-AP (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13399-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13399-1-AP (Zinc-alpha-2-glycoprotein antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 13399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13399-1-AP (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13399-1-AP (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13399-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13399-1-AP (Zinc-alpha-2-glycoprotein antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 13399-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Zinc Alpha 2 Glycoprotein antibody (13399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of PC-3 using 13399-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using Zinc Alpha 2 Glycoprotein antibody (13399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: human plasma, MCF-7 cells
• Positive IHC detected in: mouse liver tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 4 publications below

Product Information

13399-1-AP targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Zinc Alpha 2 Glycoprotein fusion protein Ag4232
• Full Name: alpha-2-glycoprotein 1, zinc-binding
• Calculated Molecular Weight: 298 aa, 34 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC033830
• Gene Symbol: Zinc Alpha 2 Glycoprotein
• Gene ID (NCBI): 563
• RRID: AB_2062582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

Publications

Species	Application	Title
human	IHC	Sci Adv; Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.; Authors - Takafumi Kato
human	IHC	Int J Cancer; Reduced AZGP1 expression is an independent predictor of early PSA recurrence and associated with ERG fusion positive and PTEN deleted prostate cancers.; Authors - Christoph Burdelski
human	IHC	J Transl Med; Decreased expression of zinc-alpha2-glycoprotein in hepatocellular carcinoma associates with poor prognosis.; Authors - Huang Yan Y
human	WB	Proteome Sci; Identification of low-abundance proteins via fractionation of the urine proteome with weak anion exchange chromatography.; Authors - Lu Chih-Ming CM
human	IHC	Obesity (Silver Spring); PMID: 24753506; Authors - Miroslav Balaz