Zinc Alpha 2 Glycoprotein Polyclonal antibody

Zinc Alpha 2 Glycoprotein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13399-1-AP

Synonyms

AZGP1, Zinc-alpha-2-glycoprotein, Zn alpha 2 GP, Zn alpha 2 glycoprotein, ZAG



Tested Applications

Positive WB detected inhuman plasma, MCF-7 cells
Positive IHC detected inmouse liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13399-1-AP targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Zinc Alpha 2 Glycoprotein fusion protein Ag4232
Full Name alpha-2-glycoprotein 1, zinc-binding
Calculated Molecular Weight 298 aa, 34 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC033830
Gene Symbol Zinc Alpha 2 Glycoprotein
Gene ID (NCBI) 563
RRIDAB_2062582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

Protocols

Product Specific Protocols
WB protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-APDownload protocol
IHC protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-APDownload protocol
IF protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Sci Adv

Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.

Authors - Takafumi Kato
humanIHC

Int J Cancer

Reduced AZGP1 expression is an independent predictor of early PSA recurrence and associated with ERG fusion positive and PTEN deleted prostate cancers.

Authors - Christoph Burdelski
humanIHC

J Transl Med

Decreased expression of zinc-alpha2-glycoprotein in hepatocellular carcinoma associates with poor prognosis.

Authors - Huang Yan Y
humanWB

Proteome Sci

Identification of low-abundance proteins via fractionation of the urine proteome with weak anion exchange chromatography.

Authors - Lu Chih-Ming CM
humanIHC

Obesity (Silver Spring)

PMID: 24753506

Authors - Miroslav Balaz