Aurora B Monoclonal antibody

Aurora B Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

3H1

Cat no : 60098-1-Ig

Synonyms

AIK2, AIM 1, AIM1, ARK 2, ARK2, AurB, AURKB, Aurora B, aurora kinase B, Aurora related kinase 2, Aurora/IPL1 related kinase 2, IPL1, STK 1, STK12, STK5

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Validation Data Gallery

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  WB analysis of HeLa using 60098-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60098-1-Ig (Aurora B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 60098-1-Ig

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 60098-1-Ig (Aurora B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 60098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60098-1-Ig (Aurora B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 60098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60098-1-Ig (Aurora B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse thymus tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

60098-1-Ig targets Aurora B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Aurora B fusion protein Ag7532
• Full Name: aurora kinase B
• Calculated Molecular Weight: 39.2 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC013300
• Gene Symbol: AURKB
• Gene ID (NCBI): 9212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aurora B(AURKB) is also named as aurora kinase B, AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5 and belongs to the Aurora subfamily. Aurora kinase B is a chromosomal passenger serine/threonine protein kinase that regulates accurate chromosomal segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachments, and regulation of the mitotic checkpoint(PMID:18311747). Expression levels of AURKB are increased in the anaplastic thyroid carcinoma cell lines ARO, Cal 62, and KAT-4(PMID:15562011).

Publications

Species	Application	Title
human	WB	Gastroenterology; Overexpression of Rac GTPase Activating Protein 1 Contributes to Proliferation of Cancer Cells by Reducing Hippo Signaling to Promote Cytokinesis.; Authors - Xiao-Mei Yang
mouse	WB	Hum Mol Genet; Downregulation of Survivin contributes to cell-cycle arrest during postnatal cardiac development in a severe spinal muscular atrophy mouse model.; Authors - Lei Sheng
human	WB	PLoS One; Anti-pancreatic cancer deliverables from sea: first-hand evidence on the efficacy, molecular targets and mode of action for multifarious polyphenols from five different brown-algae.; Authors - Aravindan Sheeja S
human	WB	FEBS J; YAP co-localizes with the mitotic spindle and midbody to safeguard mitotic division in lung-cancer cells; Authors - Shu-Er Chow