AURKA Recombinant antibody

AURKA Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230087F7

Cat no : 82906-2-RR

Synonyms

Aurora A, AURK, AURA, ARK-1, AIRK1

---

Validation Data Gallery

• 

  WB analysis using 82906-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82906-2-RR (AURKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 82906-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AURKA antibody (82906-2-RR, Clone: 230087F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  Affinity and Kinetic Characterization of 82906-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82906-2-RR against Human AURKA were performed. The affinity constant is 76.9 pM.

---

Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells, HeLa cells, NCCIT cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82906-2-RR targets AURKA in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AURKA fusion protein Ag0362
• Full Name: aurora kinase A
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46-52 kDa
• GenBank Accession Number: BC002499
• Gene Symbol: AURKA
• Gene ID (NCBI): 6790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).