AURKA Monoclonal antibody

• Featured Product
• KD/KO Validated

AURKA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

17

CloneNo.

1F4B10

Reviews (1)

Cat no : 66757-1-Ig

Synonyms

AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

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Validation Data Gallery

• 

  WB analysis of human testis using 66757-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66757-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66757-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66757-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 66757-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 66757-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66757-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66757-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66757-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AURKA antibody (66757-1-Ig, Clone: 1F4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human testis tissue, HeLa cells, NCCIT cells, rat testis tissue, mouse testis tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

66757-1-Ig targets AURKA in WB, IHC, IF/ICC, CoIP, ChIP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: AURKA fusion protein Ag20096
• Full Name: aurora kinase A
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46-52 kDa
• GenBank Accession Number: BC002499
• Gene Symbol: AURKA
• Gene ID (NCBI): 6790
• RRID: AB_2882103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Publications

Species	Application	Title
human	WB	Front Oncol; PUF60/AURKA Axis Contributes to Tumor Progression and Malignant Phenotypes in Bladder Cancer.; Authors - Qian Long
human	IHC	Front Oncol; Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression.; Authors - Bo Liang
human	WB	Front Oncol; Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities; Authors - Jing Hang ; KD Validated
human	WB	Biochem Pharmacol; Palmatine induces G2/M phase arrest and mitochondrial-associated pathway apoptosis in colon cancer cells by targeting AURKA.; Authors - Xiaojiang Liu
human	WB	Carcinogenesis; ARID3A promotes the development of colorectal cancer by upregulating AURKA.; Authors - Jing Tang
human	WB	Biochim Biophys Acta Mol Basis Dis; Blocking AURKA with MK-5108 attenuates renal fibrosis in chronic kidney disease.; Authors - Mingzhu Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah-Eve (Verified Customer) (06-02-2023)

Works well, WB dilution 1:2000

• Applications: Western Blot
• Cell Tissue Type: Human pulmonary artery smooth muscle cells