AURKA Polyclonal antibody

• Featured Product
• KD/KO Validated

AURKA Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28371-1-AP

Synonyms

Aurora A, aurora kinase A, AURK, AURA, ARK-1

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Validation Data Gallery

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  WB analysis using 28371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28371-1-AP (AURKA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 28371-1-AP

  WB result of AURKA antibody (28371-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AURKA transfected HEK-293 cells.

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  IF Staining of HeLa using 28371-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AURKA antibody (28371-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Caco-2 using 28371-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using AURKA antibody (28371-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, SW 1990 cells
• Positive IF/ICC detected in: HeLa cells, Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28371-1-AP targets AURKA in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AURKA fusion protein Ag29006
• Full Name: aurora kinase A
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC002499
• Gene Symbol: AURKA
• Gene ID (NCBI): 6790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).