ATXN3L Polyclonal antibody

ATXN3L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23166-1-AP

Synonyms

ataxin 3 like, ATX3L, ATXN3L, MJDL, Putative ataxin 3 like protein

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Validation Data Gallery

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  WB analysis of human testis using 23166-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 23166-1-AP (ATXN3L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 23166-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 23166-1-AP (ATXN3L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 23166-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23166-1-AP (ATXN3L Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 23166-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23166-1-AP (ATXN3L Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23166-1-AP is ATXN3L Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATXN3L fusion protein Ag19611
• Full Name: ataxin 3-like
• Calculated Molecular Weight: 355 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC137187
• Gene Symbol: ATXN3L
• Gene ID (NCBI): 92552
• RRID: AB_2879221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATXN3L, also named as Ataxin-3-like protein, is a 355 amino acid protein, which contains 1 Josephin domain and 2 UIM (ubiquitin-interacting motif) domains. ATXN3L localizes in the nucleus and as a deubiquitinating enzyme that cleaves both 'Lys-48'-linked and 'Lys-63'-linked poly-ubiquitin chains.